CellAdhere™ Laminin-521

与 TeSR™ 维持培养基配合使用，用于维持人胚胎干细胞（ES）和诱导多能干细胞（iPS）的基质

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产品号 #77003_C

与 TeSR™ 维持培养基配合使用，用于维持人胚胎干细胞（ES）和诱导多能干细胞（iPS）的基质

产品优势

使用含有胚胎发生过程中首个ECM蛋白的生理相关基质，模拟天然干细胞微环境
使用成分明确、无异源物质的重组人源蛋白基质，减少实验变异性
通过经过验证的常规团块传代和单细胞应用方案，支持灵活的传代工作流程
在长期培养过程中，无需添加凋亡抑制剂即可增强单细胞附着和存活
与全系列无饲养层和无血清的hPSC维持培养基TeSR™兼容

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总览

CellAdhere™ Laminin-521是一种成分明确、不含异源的重组人细胞外基质 (ECM)，可实现可靠的hPSC贴壁、扩增和分化。

CellAdhere™ Laminin-521旨在高度模拟体内干细胞微环境，其配方中含有重组人Laminin-521，这是哺乳动物胚胎发育过程中最早表达的ECM蛋白之一，也是胚胎内细胞团中hPSC的天然分泌物。通过在体外重现这种天然环境，CellAdhere™ Laminin-521提供了一种高度稳定且具有生理相关性的基质，可促进高效的整合素介导的细胞贴壁、一致的细胞增殖以及稳定的细胞群，从而在下游应用中获得可重复的结果。

CellAdhere™ Laminin-521支持团块传代和单细胞传代方案。与任何TeSR™维持培养基搭配使用时，它支持可靠的高密度团块传代，从而简化标准扩增流程。此外，它还能增强单细胞贴壁和克隆存活率。CellAdhere™ Laminin-521与eTeSR™配合使用时，长期培养过程中可无需使用凋亡抑制剂（例如ROCK抑制剂）。

在CellAdhere™ Laminin-521上培养的人多能干细胞 (hPSC) 可保持其特征形态，表达未分化细胞标志物（例如OCT4和TRA-1-60），并维持分化为所有三个胚层（外胚层、中胚层和内胚层）的能力。

这种成分明确的基质具有极佳的灵活性，并且与无饲养层和无血清的维持培养基完全兼容，包括mTeSR™1、mTeSR™ Plus、TeSR™-E8™、TeSR™-AOF和eTeSR™。

实验数据

Figure 1. Morphology of hiPSCs Cultured on CellAdhere™ Laminin-521 in mTeSR™ Plus

Human induced pluripotent stem cells (hiPSCs; SCTi004-A) were maintained on CellAdhere™ Laminin-521 in mTeSR™ Plus and imaged on Day 7. Cultures formed compact, densely packed colonies with defined edges and characteristic hiPSC morphology. Representative images are shown at 2X and 10X magnification.

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CellAdhere™ Laminin-521

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CellAdhere™ Laminin-521

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77003

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English

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CellAdhere™ Laminin-521

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200-0117

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English

应用领域

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文献 (12)

Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Draper JS et al. Nature biotechnology 2004 JAN

Abstract

We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.

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Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Amps K et al. Nature biotechnology 2011 DEC

Abstract

The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines,from 38 laboratories worldwide,for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal,but there was a progressive tendency to acquire changes on prolonged culture,commonly affecting chromosomes 1,12,17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants,determined from the SNP arrays,also appeared sporadically. No common variants related to culture were observed on chromosomes 1,12 and 17,but a minimal amplicon in chromosome 20q11.21,including three genes expressed in human ES cells,ID1,BCL2L1 and HM13,occurred in textgreater20% of the lines. Of these genes,BCL2L1 is a strong candidate for driving culture adaptation of ES cells.

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A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells Lu HF et al. Biomaterials 2014 MAR

Abstract

A defined xeno-free system for patient-specific iPSC derivation and differentiation is required for translation to clinical applications. However,standard somatic cell reprogramming protocols rely on using MEFs and xenogeneic medium,imposing a significant obstacle to clinical translation. Here,we describe a well-defined culture system based on xeno-free media and LN521 substrate which supported i) efficient reprogramming of normal or diseased skin fibroblasts from human of different ages into hiPSCs with a 15-30 fold increase in efficiency over conventional viral vector-based method; ii) long-term self-renewal of hiPSCs; and iii) direct hiPSC lineage-specific differentiation. Using an excisable polycistronic vector and optimized culture conditions,we achieved up to 0.15%-0.3% reprogramming efficiencies. Subsequently,transgene-free hiPSCs were obtained by Cre-mediated excision of the reprogramming factors. The derived iPSCs maintained long-term self-renewal,normal karyotype and pluripotency,as demonstrated by the expression of stem cell markers and ability to form derivatives of three germ layers both in vitro and in vivo. Importantly,we demonstrated that Parkinson's patient transgene-free iPSCs derived using the same system could be directed towards differentiation into dopaminergic neurons under xeno-free culture conditions. Our approach provides a safe and robust platform for the generation of patient-specific iPSCs and derivatives for clinical and translational applications. textcopyright 2013 Elsevier Ltd.

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CellAdhere™ Laminin-521

产品号 #77003

产品号 #200-0117

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产品号 #77003_C

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CellAdhere™ Laminin-521

产品号 #77003

产品号 #200-0117

产品号 #（选择产品）

产品号 #77003_C

产品优势

总览

实验数据

产品说明书及文档

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