EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07920_C
细胞分离溶液
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总览
ACCUTASE™ 适用于常规细胞从塑料器皿上的分离操作。这是一种含有蛋白水解酶和胶原酶活性的即用型溶液，已被验证可广泛适用于多种细胞类型。相比传统胰蛋白酶，ACCUTASE™ 浓度较低，更加温和，有助于保留细胞表面表位，适用于后续流式细胞术分析，并确保高细胞活力，无需使用中和溶液。ACCUTASE™ 不含哺乳动物或细菌来源成分。每一批产品均经过以下检测：无菌性（采用 USP 膜过滤法）、酶活性（使用合成的显色四肽检测）以及细胞从组织培养塑料上的分离测试。
包含
• 含1X ACCUTASE™酶的杜氏磷酸盐缓冲液 (PBS)
• 0.5 mM EDTA
• 4Na
• 3 mg/L酚红
分类
酶法相关（或酶解类产品）
细胞类型
神经细胞，PSC衍生，多能干细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
细胞培养
研究领域
神经科学，干细胞生物学
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (4)
文献 (53)
A Model to Evaluate the Cytotoxicity of the Fungal Volatile Organic Compound 1-octen-3-ol in Human Embryonic Stem Cells
Mycopathologia 2012 JAN
Abstract
Microbial growth in damp indoor environments has been correlated with risks to human health. This study was aimed to determine the cytotoxicity of 1-octen-3-ol (mushroom alcohol")�
Suspension bioreactor expansion of undifferentiated human embryonic stem cells
Methods in molecular biology (Clifton,N.J.) 2012 JAN
Abstract
Embryonic stem cells (ESCs) are unique cells,which have the ability to differentiate into all cell types that comprise the adult organism. Furthermore,ESCs can infinitely self-renew under optimized conditions. These features place human ESCs (hESCs) in a position where these cells can be exploited for tissue engineering and regenerative medicine approaches in treating human degenerative disorders. However,cell therapy approaches will require large amounts of clinically useable cells,not typically achievable using standard static cell culture methods. Here,we describe a method wherein clinically relevant numbers of hESCs can be generated in a cost and time effective manner.
Signaling defects in iPSC-derived fragile X premutation neurons
Human Molecular Genetics 2012 SEP
Abstract
Fragile X-associated tremor/ataxia syndrome (FXTAS) is a leading monogenic neurodegenerative disorder affecting premutation carriers of the fragile X (FMR1) gene. To investigate the underlying cellular neuropathology,we produced induced pluripotent stem cell-derived neurons from isogenic subclones of primary fibroblasts of a female premutation carrier,with each subclone bearing exclusively either the normal or the expanded (premutation) form of the FMR1 gene as the active allele. We show that neurons harboring the stably-active,expanded allele (EX-Xa) have reduced postsynaptic density protein 95 protein expression,reduced synaptic puncta density and reduced neurite length. Importantly,such neurons are also functionally abnormal,with calcium transients of higher amplitude and increased frequency than for neurons harboring the normal-active allele. Moreover,a sustained calcium elevation was found in the EX-Xa neurons after glutamate application. By excluding the individual genetic background variation,we have demonstrated neuronal phenotypes directly linked to the FMR1 premutation. Our approach represents a unique isogenic,X-chromosomal epigenetic model to aid the development of targeted therapeutics for FXTAS,and more broadly as a model for the study of common neurodevelopmental (e.g. autism) and neurodegenerative (e.g. Parkinsonism,dementias) disorders.
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Contains | • 1X ACCUTASE™ enzymes in Dulbecco’s phosphate-buffered saline (PBS) • 0.5 mM EDTA•4Na • 3 mg/L Phenol red |
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法律声明:
ACCUTASE is a trademark of Innovative Cell Technologies, Inc. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
ACCUTASE is a trademark of Innovative Cell Technologies, Inc. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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