EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05990_C
无饲养层、无动物成分的人胚胎干细胞和诱导多能干细胞维持培养基
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总览
TeSR™-E8™ 是一款无饲养层、无动物成分的培养基，适用于培养人胚胎干细胞 (ES) 和人诱导性多能干细胞 (iPS)。它基于威斯康星大学麦迪逊分校 James Thomson 博士实验室研发的 E8 配方，该实验室是 mTeSR™1 研发的主导研发团队，mTeSR™1 是目前发表论文中使用最广泛的多能干细胞无饲养层培养基。
与 TeSR™ 产品家族中的其他产品一样，TeSR™-E8™ 培养基以最高标准和严谨工艺生产。该培养基专门研发，仅包含维持 ESCs 和 iPSCs 生长所需的必需成分，是多能干细胞培养中最简化的培养基。TeSR™-E8™ 可搭配 Corning® Matrigel® hESC-Qualified Matrix（Corning 354277）一起使用，若需完全明确的无异源成分系统，可选用 Vitronectin XF™（产品号 #07180）或 Laminin-521（产品号 #77003）作为培养基质。
分类
专用培养基
细胞类型
多能干细胞
种属
人
应用
细胞培养，扩增，培养
品牌
TeSR
研究领域
干细胞生物学
制剂类别
无动物成分，化学成分明确，无血清
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (19)
文献 (53)
Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States.
Cell stem cell 2017 MAR
Abstract
Human pluripotent stem cells (PSCs) exist in naive and primed states and provide important models to investigate the earliest stages of human development. Naive cells can be obtained through primed-to-naive resetting,but there are no reliable methods to prospectively isolate unmodified naive cells during this process. Here we report comprehensive profiling of cell surface proteins by flow cytometry in naive and primed human PSCs. Several naive-specific,but not primed-specific,proteins were also expressed by pluripotent cells in the human preimplantation embryo. The upregulation of naive-specific cell surface proteins during primed-to-naive resetting enabled the isolation and characterization of live naive cells and intermediate cell populations. This analysis revealed distinct transcriptional and X chromosome inactivation changes associated with the early and late stages of naive cell formation. Thus,identification of state-specific proteins provides a robust set of molecular markers to define the human PSC state and allows new insights into the molecular events leading to naive cell resetting.
Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy.
Stem cell research 2017
Abstract
Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 62-year-old female with familial hypertrophic cardiomyopathy (HCM). PBMCs were reprogrammed to a pluripotent state following transfection with non-integrative episomal vectors carrying reprogramming factors OCT4,SOX2,LIN28,KLF4 and L-MYC. iPSCs were shown to express pluripotency markers,possess trilineage differentiation potential,carry rare variants identified in DNA isolated directly from the patient's whole blood,have a normal karyotype and no longer carry episomal vectors for reprogramming. This line is a useful resource for identifying unknown genetic causes of HCM.
Generation of non-transgenic iPS cells from human cord blood CD34+ cells under animal component-free conditions.
Stem cell research 2017
Abstract
Recently,many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells,reprogrammed using CytoTune™ Sendai reprogramming vectors. CD34+ cell isolation,cultivation,reprogramming and establishment of resulting hiPSC lines were performed under the exclusive usage of animal-derived component-free (ADCF) materials and components.
更多信息
| 物种 | 人 |
|---|---|
| 配方 | 不含动物成分 |
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法律声明:
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