EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05946_C
成分明确、无血清、无异种成分的多能干细胞培养基
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总览
TeSR™-E6 是一种成分明确、无血清且无异种成分的培养基，基于 TeSR™-E8™ 的配方，但不含转化生长因子 β（TGF-β）和碱性成纤维细胞生长因子（bFGF）)。它可用作人胚胎干细胞 (ES) 和诱导多能干细胞 (iPS) 分化的基础培养基，或用于其他需要去除上述细胞因子的应用。
分类
专用培养基
细胞类型
多能干细胞
种属
人
应用
细胞培养，鉴定，分化
品牌
TeSR
研究领域
干细胞生物学
制剂类别
无血清，无异源
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (5)
文献 (8)
α-Ketoglutarate Accelerates the Initial Differentiation of Primed Human Pluripotent Stem Cells.
Cell metabolism 2016 SEP
Abstract
Pluripotent stem cells (PSCs) can self-renew or differentiate from naive or more differentiated,primed,pluripotent states established by specific culture conditions. Increased intracellular α-ketoglutarate (αKG) was shown to favor self-renewal in naive mouse embryonic stem cells (mESCs). The effect of αKG or αKG/succinate levels on differentiation from primed human PSCs (hPSCs) or mouse epiblast stem cells (EpiSCs) remains unknown. We examined primed hPSCs and EpiSCs and show that increased αKG or αKG-to-succinate ratios accelerate,and elevated succinate levels delay,primed PSC differentiation. αKG has been shown to inhibit the mitochondrial ATP synthase and to regulate epigenome-modifying dioxygenase enzymes. Mitochondrial uncoupling did not impede αKG-accelerated primed PSC differentiation. Instead,αKG induced,and succinate impaired,global histone and DNA demethylation in primed PSCs. The data support αKG promotion of self-renewal or differentiation depending on the pluripotent state.
C9ORF72 patient-derived endothelial cells drive blood-brain barrier disruption and contribute to neurotoxicity
Fluids and Barriers of the CNS 2024 Apr
Abstract
The blood-brain barrier (BBB) serves as a highly intricate and dynamic interface connecting the brain and the bloodstream,playing a vital role in maintaining brain homeostasis. BBB dysfunction has been associated with multiple neurodegenerative diseases,including amyotrophic lateral sclerosis (ALS); however,the role of the BBB in neurodegeneration is understudied. We developed an ALS patient-derived model of the BBB by using cells derived from 5 patient donors carrying C9ORF72 mutations. Brain microvascular endothelial-like cells (BMEC-like cells) derived from C9ORF72-ALS patients showed altered gene expression,compromised barrier integrity,and increased P-glycoprotein transporter activity. In addition,mitochondrial metabolic tests demonstrated that C9ORF72-ALS BMECs display a significant decrease in basal glycolysis accompanied by increased basal and ATP-linked respiration. Moreover,our study reveals that C9-ALS derived astrocytes can further affect BMECs function and affect the expression of the glucose transporter Glut-1. Finally,C9ORF72 patient-derived BMECs form leaky barriers through a cell-autonomous mechanism and have neurotoxic properties towards motor neurons.Graphical Abstract Supplementary InformationThe online version contains supplementary material available at 10.1186/s12987-024-00528-6.
Multiplexed bulk and single-cell RNA-seq hybrid enables cost-efficient disease modeling with chimeric organoids
Nature Communications 2024 May
Abstract
Disease modeling with isogenic Induced Pluripotent Stem Cell (iPSC)-differentiated organoids serves as a powerful technique for studying disease mechanisms. Multiplexed coculture is crucial to mitigate batch effects when studying the genetic effects of disease-causing variants in differentiated iPSCs or organoids,and demultiplexing at the single-cell level can be conveniently achieved by assessing natural genetic barcodes. Here,to enable cost-efficient time-series experimental designs via multiplexed bulk and single-cell RNA-seq of hybrids,we introduce a computational method in our Vireo Suite,Vireo-bulk,to effectively deconvolve pooled bulk RNA-seq data by genotype reference,and thereby quantify donor abundance over the course of differentiation and identify differentially expressed genes among donors. Furthermore,with multiplexed scRNA-seq and bulk RNA-seq,we demonstrate the usefulness and necessity of a pooled design to reveal donor iPSC line heterogeneity during macrophage cell differentiation and to model rare WT1 mutation-driven kidney disease with chimeric organoids. Our work provides an experimental and analytic pipeline for dissecting disease mechanisms with chimeric organoids. IPSC-derived organoids model diseases. Multiplexed coculture and demultiplexing natural genetic barcodes aid in studying genetic effects. Here,authors introduce Vireo-bulk to deconvolve bulk RNA-seq data,quantify donor abundance and identify differentially expressed genes.
更多信息
| 物种 | 人 |
|---|---|
| 配方 | 无血清 |
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本产品系基于WiCell™研究院（WiCell™ Research Institute）知识产权授权许可开发。本产品的出售目的仅限于基于不可转让、用途受限之许可下的研究用途（无论购买者为学术机构或营利性主体）。购买本产品并不被授予为商业用途（即，以获利为目的的任何行为，如将本产品用于制造用途、或转售本产品或使用本产品所制成的任何材料、或将本产品或使用本产品所制成的材料用于提供服务）或临床应用（即，将本产品或本产品所用材料应用于人体）而出售、使用或另行转让本产品的权利，或出于基础临床前研究应用（包括但不限于畸胎瘤试验）以外的目的，由营利性主体或与其合作，将使用本产品制造的任何材料植入动物体内的权利。
购买者若不同意前述条款和条件，应保持产品完好，将其退还出售方以获退款。
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产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
本产品系基于WiCell™研究院（WiCell™ Research Institute）知识产权授权许可开发。本产品的出售目的仅限于基于不可转让、用途受限之许可下的研究用途（无论购买者为学术机构或营利性主体）。购买本产品并不被授予为商业用途（即，以获利为目的的任何行为，如将本产品用于制造用途、或转售本产品或使用本产品所制成的任何材料、或将本产品或使用本产品所制成的材料用于提供服务）或临床应用（即，将本产品或本产品所用材料应用于人体）而出售、使用或另行转让本产品的权利，或出于基础临床前研究应用（包括但不限于畸胎瘤试验）以外的目的，由营利性主体或与其合作，将使用本产品制造的任何材料植入动物体内的权利。
购买者若不同意前述条款和条件，应保持产品完好，将其退还出售方以获退款。
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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