TeSR™ AOF 3D

适用于人胚胎干细胞（hESC）和诱导多能干细胞（hiPSC）的无动物源成分悬浮培养基

只有 %1 左

率先评论此产品

¥10,148.00

产品号 #（选择产品）

产品号 #100-0720_C

适用于人胚胎干细胞（hESC）和诱导多能干细胞（hiPSC）的无动物源成分悬浮培养基

产品优势

无动物来源的原材料可追溯到二级生产原材料水平，从而提高安全性。
无需全培养基换液的分批补给培养策略节省了时间和劳动力。
快速扩增高质量的hPSCs，无需2D培养转为3D培养的驯化过程。

产品组分包括

TeSR™-AOF 3D接种基础培养基（480 mL）
TeSR™-AOF 3D接种补充剂（20 mL）
TeSR™-AOF 3D补给补充剂（20 mL）

立即询价

Need a high-quality cell source? Use the hiPSC SCTi003-A (female) or SCTi004-A (male) control lines, manufactured with mTeSR™ Plus.

总览

实验数据

产品说明书及文档

应用领域

总览

TeSR™-AOF 3D是一种无动物源成分培养基，专为人胚胎干细胞（hESC）和诱导多能干细胞（hiPSC）在3D悬浮培养中以聚集体形式扩增及规模化培养而开发。基于TeSR™-AOF配方，TeSR™-AOF 3D培养基性能稳定，其优化的分批补给方案适用于悬浮培养中规模化生产人胚胎干细胞（hESC）和诱导多能干细胞（hiPSC）。创新的分批补给流程节省时间和培养基；每日补给可补充营养，无需在非传代日更换培养基。

TeSR™-AOF 3D系统兼容以下基质预培养的细胞：mTeSR™1（产品号 #85850）、mTeSR™ Plus（产品号 #100-0276）、TeSR™-AOF（产品号 #100-0401）或TeSR™-E8™（产品号 #05990），培养基质包括Corning® Matrigel® hESC-Qualified Matrix（Corning 产品号 #354277）、Vitronectin XF™（产品号 #07180）或CellAdhere™ Laminin-521（产品号 #77003）。与多种悬浮培养容器兼容。

该培养基及其组成成分的生产过程中，至少在二级生产原材料水平不含任何动物或人源成分。TeSR™-AOF 3D 在二级生产原材料上无动物成分。每批用于配制TeSR™-AOF完全培养基的TeSR™-AOF 3D接种补充剂和TeSR™-AOF 3D补给补充剂经过性能测试，并在培养实验中使用人多能干细胞进行验证。

分类
专用培养基

细胞类型
多能干细胞

应用
细胞培养

品牌
TeSR

研究领域
疾病建模，干细胞生物学，细胞治疗开发

制剂类别
无动物源

查看更多显示更少

实验数据

Figure 1. Growth of Human Pluripotent Stem Cells (hPSCs) in TeSR™-AOF 3D

TeSR™-AOF 3D supports the expansion and high viability of hPSCs over multiple passages in aggregate suspension culture. Shown are (A) cumulative viable cells, (B) daily fold expansion, and (C) end-of-passage viabilities in human ES cell lines (H1 and H7) and human iPS cell lines (WLS-1C and STiPS-F016) over 5 passages. Error bars represent ± SD, n = 3.

Figure 2. hPSCs Cultured in TeSR™-AOF 3D Express Markers of the Undifferentiated State and Differentiate to the Three Germ Layers

Shown are (A) OCT-4 and TRA-1-60 expression and (B) trilineage differentiation potential (ectoderm: % Nestin+/Pax6+, mesoderm: % Brach+, and endoderm: % Sox-17+/CXCR4+) using the STEMdiff™ Trilineage Differentiation Kit for multiple hPSC lines after 5 passages in TeSR™-AOF 3D. Error bars represent ± SD, n = 3.

Figure 3. Metabolic Profile of hPSCs During Culture in TeSR™-AOF 3D

(A) Glucose concentration in TeSR™-AOF 3D is maintained at optimal levels throughout the culture period. Dotted line represents the glucose level in TeSR™-AOF 3D Complete Seed Medium. (B) Lactate concentration is maintained at low levels (below 15 mM as indicated by the dotted line) throughout the culture period for multiple hPSC lines after 5 passages in TeSR™-AOF 3D. Error bars represent ± SD, n = 3.

Figure 4. Morphology of hPSC Aggregates in TeSR™-AOF 3D

Multiple hPSC lines maintained in TeSR™-AOF 3D depict morphology characteristic of hPSC aggregates in suspension culture, namely a mostly spherical shape, clear edges (not smooth or shiny), even color, a dimpled or pockmarked appearance, and are less than 400 µm in size.