StemSpan™-ACF红系扩增培养基

用于培养和扩增人红系细胞的无动物成分培养基

只有 %1 左

率先评论此产品

¥9,420.00

产品号 #（选择产品）

产品号 #09860_C

用于培养和扩增人红系细胞的无动物成分培养基

产品优势

专为红系祖细胞扩增和分化设计的无动物成分培养条件
为搭配使用StemSpan™红系扩增添加物而优化
可从每个起始CD34+细胞产生数千个CD71+GlyA+红系细胞

产品组分包括

专为红系祖细胞扩增和分化设计的无动物成分培养条件
为搭配使用StemSpan™红系扩增添加物而优化
可从每个起始CD34+细胞产生数千个CD71+GlyA+红系细胞

立即询价

总览

StemSpan™-ACF红系扩增培养基（ACF-E）是一种无动物成分（ACF）培养基，用于人造血细胞的体外培养和扩增。当搭配使用StemSpan™红系扩增添加物（100X; 产品号 #02692）时，该培养基可在ACF培养条件下支持人造血祖细胞扩增并分化为CD71+和血型糖蛋白A+（GlyA+）的成红细胞。该培养基仅包含重组蛋白和合成成分，不含血清或其他人或动物来源的成分。

实验数据

Figure 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

(A) Average numbers of erythroid cells generated after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM (black bars), SFEM II (grey bars), or StemSpan™-ACF Erythroid Expansion Medium (ACF-E, orange bars) media containing StemSpan™ Erythroid Expansion Supplement (Catalog #02692). Shown are the number of erythroid cells that express CD71 and/or Glycophorin A (GlyA) per input CD34+ cell. (B) The percentages of the different erythroid cell subsets generated in these cultures are shown, including CD71+GlyA+ erythroblasts, immature CD71+GlyA- erythroid progenitor cells and pro-erythroblasts, and CD71-/lowGlyA+ normoblasts. All three media supported the generation of thousands of erythroid cells per CB-derived CD34+ cell plated.

Table 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

Yields and percentages of erythroid cells produced after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM, SFEM II or ACF-E media containing Erythroid Expansion Supplement. Erythroid cells were identified by flow cytometry after staining with antibodies against CD71 and GlyA. The % erythroid cells represent the percentage of cells that express CD71 and/or GlyA.

*CI: Confidence Interval.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

文献 (1)

Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks B. Yuan et al. BMC Biology 2024 Apr

Abstract

CRISPR-Cas9 genome editing often induces unintended,large genomic rearrangements,posing potential safety risks. However,there are no methods for mitigating these risks. Using long-read individual-molecule sequencing (IDMseq),we found the microhomology-mediated end joining (MMEJ) DNA repair pathway plays a predominant role in Cas9-induced large deletions (LDs). We targeted MMEJ-associated genes genetically and/or pharmacologically and analyzed Cas9-induced LDs at multiple gene loci using flow cytometry and long-read sequencing. Reducing POLQ levels or activity significantly decreases LDs,while depleting or overexpressing RPA increases or reduces LD frequency,respectively. Interestingly,small-molecule inhibition of POLQ and delivery of recombinant RPA proteins also dramatically promote homology-directed repair (HDR) at multiple disease-relevant gene loci in human pluripotent stem cells and hematopoietic progenitor cells. Our findings reveal the contrasting roles of RPA and POLQ in Cas9-induced LD and HDR,suggesting new strategies for safer and more precise genome editing. The online version contains supplementary material available at 10.1186/s12915-024-01896-z.

View publication

更多信息

更多信息
物种	人
Contains	This product contains only recombinant proteins and synthetic components
配方	不含动物成分, 无血清

StemSpan™-ACF红系扩增培养基

产品号 #09860

产品号 #（选择产品）

产品号 #09860_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

Request Pricing

更多信息

Scientists Helping Scientists™

StemSpan™-ACF红系扩增培养基

产品号 #09860

产品号 #（选择产品）

产品号 #09860_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (5)

文献 (1)

Abstract

Request Pricing

更多信息

欢迎您关注STEMCELL Technologies的微信公众平台