EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05230_C
功能检测试剂盒，用于评估人ES和iPS细胞定向分化至三个胚层的多能性
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总览
STEMdiff™ 三谱系分化试剂盒提供了一种简单的培养检测方法，用于功能性地验证新建或已建立的人胚胎干细胞 (ES) 和诱导多能干细胞 (iPS) 系分化为三个胚层（外胚层、中胚层和内胚层）的能力。该试剂盒包含专门的完整培养基和基于单层细胞的实验方案，可对每个胚层进行平行的体外定向分化实验，并在一周内清晰且可重复地确认三胚层分化潜能。STEMdiff™ 三谱系分化试剂盒设计为终点检测用途，不适用于后续分化或其他应用所需细胞的制备。STEMdiff™ 三谱系分化试剂盒设计为终点检测用途，不适用于后续分化或其他应用所需细胞的制备。本试剂盒已针对在 mTeSR™1、mTeSR™ Plus 或 TeSR™-AOF 培养的细胞进行了优化。
分类
专用培养基
细胞类型
多能干细胞
种属
人
应用
细胞培养，鉴定，分化，功能学筛选，表型鉴定
品牌
STEMdiff
研究领域
干细胞生物学
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (33)
文献 (40)
Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy.
Stem cell research 2017
Abstract
Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 62-year-old female with familial hypertrophic cardiomyopathy (HCM). PBMCs were reprogrammed to a pluripotent state following transfection with non-integrative episomal vectors carrying reprogramming factors OCT4,SOX2,LIN28,KLF4 and L-MYC. iPSCs were shown to express pluripotency markers,possess trilineage differentiation potential,carry rare variants identified in DNA isolated directly from the patient's whole blood,have a normal karyotype and no longer carry episomal vectors for reprogramming. This line is a useful resource for identifying unknown genetic causes of HCM.
Generation of induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient with the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7) gene.
Stem cell research 2017
Abstract
Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the whole blood of a 43-year-old male with hypertrophic cardiomyopathy (HCM) who carries the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7). Patient-derived PBMCs were reprogrammed using non-integrative episomal vectors containing reprogramming factors OCT4,SOX2,LIN28,KLF4 and L-MYC. iPSCs were shown to express pluripotent markers,have trilineage differentiation potential,carry the pathogenic MYH7 variant p.Val698Ala,have a normal karyotype and no longer carry the episomal reprogramming vector. This line is useful for studying the link between variants in MYH7 and the pathogenesis of HCM.
Feeder-Free Derivation of Naïve Human Pluripotent Stem Cells.
Stem cells and development 2017 MAY
Abstract
Human pluripotent stem cells (HPSCs) cultured in conditions that maintain pluripotency via FGF and TGFβ signaling have been described as being in a primed state. These cells have been shown to exhibit characteristics more closely related to mouse epiblast-derived stem cells than to so called naïve mouse PSCs said to possess a more ground state pluripotency that mimics the early mouse embryo inner cell mass. Initial attempts to create culture conditions favorable for generation of naïve HPSCs from primed HPSCs has required the use of mouse embryonic fibroblasts as a feeder layer to support this transition. A protocol for the routine derivation and maintenance of naïve HPSCs in completely defined conditions is highly desirable for stem cell researchers to enhance the study and clinical translation of naïve HPSCs. Here we describe a standard protocol for transitioning primed HPSCs to a naïve state using commercial RSet media and xeno-free recombinant vitronectin.
更多信息
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