EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #15128_C
为血浆和密度梯度离心液的交界界面中高度富集的细胞。
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总览
RosetteSep™人间充质干细胞富集抗体混合物通过负选从新鲜骨髓分离间充质干细胞。四聚体抗体复合物可识别CD3、CD14、CD19、CD38、CD66b以及红细胞（RBC）上的糖蛋白A，从而靶向去除非目的细胞。使用密度梯度离心液如Lymphoprep™（产品号 #18060）离心后 ，非目的细胞会与红细胞一起沉淀。纯化的间充质干细胞为血浆和密度梯度离心液的交界界面中高度富集的细胞。
分类
细胞分选试剂盒
细胞类型
间充质干/祖细胞
种属
人
样本来源
骨髓
分选方法
负选
应用
细胞分选
品牌
RosetteSep
研究领域
药物发现和毒性检测，干细胞生物学
产品说明书及文档
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应用领域
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相关材料与文献
技术资料 (4)
常见问题
文献 (8)
Transfusion medicine illustrated. The mesenchymal stem cell revealed.
Transfusion 2003 JAN
Abstract
Isolation of multipotent mesenchymal stem cells from umbilical cord blood.
Blood 2004 MAR
Abstract
It is well accepted that umbilical cord blood has been a source for hematopoietic stem cells. However,controversy exists as to whether cord blood can serve as a source of mesenchymal stem cells,which can differentiate into cells of different connective tissue lineages such as bone,cartilage,and fat,and little success has been reported in the literature about the isolation of such cells from cord blood. Here we report a novel method to obtain single cell-derived,clonally expanded mesenchymal stem cells that are of multilineage differentiation potential by negative immunoselection and limiting dilution. The immunophenotype of these clonally expanded cells is consistent with that reported for bone marrow mesenchymal stem cells. Under appropriate induction conditions,these cells can differentiate into bone,cartilage,and fat. Surprisingly,these cells were also able to differentiate into neuroglial- and hepatocyte-like cells under appropriate induction conditions and,thus,these cells may be more than mesenchymal stem cells as evidenced by their ability to differentiate into cell types of all 3 germ layers. In conclusion,umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical waste. It can serve as an alternative source of mesenchymal stem cells to bone marrow.
Contribution of human bone marrow stem cells to individual skeletal myotubes followed by myogenic gene activation.
Experimental cell research 2005 JUL
Abstract
Much attention is focused on characterizing the contribution of bone marrow (BM)-derived cells to regenerating skeletal muscle,fuelled by hopes for stem cell-mediated therapy of muscle degenerative diseases. Though physical integration of BM stem cells has been well documented,little evidence of functional commitment to myotube phenotype has been reported. This is due to the innate difficulty in distinguishing gene products derived from donor versus host nuclei. Here,we demonstrate that BM-derived stem cells contribute via gene expression following incorporation to skeletal myotubes. By co-culturing human BM-derived mesenchymal stem cells (MSC) with mouse skeletal myoblasts,physical incorporation was observed by genetic lineage tracing and species-specific immunofluorescence. We used a human-specific antibody against the intermediate filament protein nestin,a marker of regenerating skeletal muscle,to identify functional contribution of MSC to myotube formation. Although nestin expression was never detected in MSC,human-specific expression was detected in myotubes that also contained MSC-derived nuclei. This induction of gene expression following myotube integration suggests that bone marrow-derived stem cells can reprogram and functionally contribute to the muscle cell phenotype. We propose that this model of myogenic commitment may provide the means to further characterize functional reprogramming of MSC to skeletal muscle.
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