EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05872_C
cGMP级、无酶的人多能干细胞选择与传代试剂
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总览
使用 ReLeSR™ 可高效地将人胚胎干细胞（ES）或诱导多能干细胞（iPS）以细胞聚集体形式进行温和解离与传代，无需手动选择或使用ReLeSR™刮取。使用 ReLeSR™ 传代人ES/iPS细胞，可轻松生成大小合适的聚集体，同时避免手动操作带来的麻烦和差异性。由于无需手动刮取，该无酶试剂适用于培养瓶及其他封闭式培养容器，有利于细胞培养的规模化和自动化。。ReLeSR™ 遵循相关的 cGMP 标准，在经过认证的质量管理体系下生产，以确保最高的质量和一致性，从而获得可重复的结果。
如需申请 ReLeSR 药品主文件 (DMF) 的授权书 (LOA) 请点击此处.
分类
非酶法
细胞类型
多能干细胞
种属
人
应用
细胞培养
品牌
ReLeSR，TeSR
研究领域
干细胞生物学
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (18)
文献 (197)
A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells.
Methods in molecular biology (Clifton,N.J.) 2016 FEB
Abstract
Human embryonic stem cells hold great promise for future biomedical applications such as disease modeling and regenerative medicine. However,these cells are notoriously difficult to culture and are refractory to common means of genetic manipulation,thereby limiting their range of applications. In this protocol,we present an easy and robust method of gene repression in human embryonic stem cells using lipofection of small interfering RNA (siRNA).
Cell-fate determination by ubiquitin-dependent regulation of translation
Nature 2015 SEP
Abstract
Metazoan development depends on the accurate execution of differentiation programs that allow pluripotent stem cells to adopt specific fates. Differentiation requires changes to chromatin architecture and transcriptional networks,yet whether other regulatory events support cell-fate determination is less well understood. Here we identify the ubiquitin ligase CUL3 in complex with its vertebrate-specific substrate adaptor KBTBD8 (CUL3(KBTBD8)) as an essential regulator of human and Xenopus tropicalis neural crest specification. CUL3(KBTBD8) monoubiquitylates NOLC1 and its paralogue TCOF1,the mutation of which underlies the neurocristopathy Treacher Collins syndrome. Ubiquitylation drives formation of a TCOF1-NOLC1 platform that connects RNA polymerase I with ribosome modification enzymes and remodels the translational program of differentiating cells in favour of neural crest specification. We conclude that ubiquitin-dependent regulation of translation is an important feature of cell-fate determination.
Defined three-dimensional microenvironments boost induction of pluripotency
Nature Materials 2016 MAR
Abstract
Since the discovery of induced pluripotent stem cells (iPSCs),numerous approaches have been explored to improve the original protocol,which is based on a two-dimensional (2D) cell-culture system. Surprisingly,nothing is known about the effect of a more biologically faithful 3D environment on somatic-cell reprogramming. Here,we report a systematic analysis of how reprogramming of somatic cells occurs within engineered 3D extracellular matrices. By modulating microenvironmental stiffness,degradability and biochemical composition,we have identified a previously unknown role for biophysical effectors in the promotion of iPSC generation. We find that the physical cell confinement imposed by the 3D microenvironment boosts reprogramming through an accelerated mesenchymal-to-epithelial transition and increased epigenetic remodelling. We conclude that 3D microenvironmental signals act synergistically with reprogramming transcription factors to increase somatic plasticity.
更多信息
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THIS PRODUCT IS MANUFACTURED AND TESTED FOLLOWING RELEVANT CGMPs UNDER A CERTIFIED QUALITY MANAGEMENT SYSTEM. PRODUCT IS FOR FURTHER MANUFACTURING OR RESEARCH USE. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. 欲获悉更多关于STEMCELL的质控信息，请访问 WWW.STEMCELL.COM/COMPLIANCE
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