产品号 #85850_C
cGMP标准、无饲养层的hESC和iPSC维持培养基
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cGMP标准、无饲养层的hESC和iPSC维持培养基
cGMP标准、无饲养层的hESC和iPSC维持培养基
cGMP标准、无饲养层的hESC和iPSC维持培养基
cGMP标准、无饲养层的hESC和iPSC维持培养基
It makes me proud knowing that my work is critical to keeping thousands of hPSC lines reliably healthy and consistent around the world.
使用这种专用的无饲养层培养基,可以获得具有均质、未分化表型的更一致的人多能干细胞 (hPSC) 培养物。
mTeSR™1 在符合相关cGMP(现行药品生产质量管理规范)的条件下生产,确保最高质量与一致性,为基础研究、细胞治疗及新药研发(IND)等应用提供可重复的实验结果。该培养基为无血清的完整细胞培养体系,采用预筛选的原材料制备,以确保批次间的一致性和在无饲养层hPSC培养中的稳定性能。
作为全球发表文献最多的无饲养层hPSC培养基,mTeSR™1 可以采用既定的方案应用于从建系到分化等各种实验流程,已被世界领先的多能干细胞研究人员用于在50多个国家成功维持数千株hPSC系。若您需要提升细胞性能并获得更灵活的细胞维持方案,也可关注 mTeSR™ Plus 培养基。该产品同样遵循相关cGMP标准生产,具有稳定组分和增强的缓冲能力。
如需申请 mTeSR™1 的 FDA 主文件的授权书 (LOA),请单击此处。
分类
专用培养基
细胞类型
多能干细胞
种属
人
应用
细胞培养,扩增,培养
品牌
TeSR
研究领域
干细胞生物学
制剂类别
无血清
Figure 1. Normal hES and hiPS Cell Morphology is Observed in cGMP mTeSR™1 Cultures
Undifferentiated (A) H1 human embryonic stem (hES) and (B) WLS-1C human induced pluripotent stem (hiPS) cells cultured on Corning® Matrigel® Matrix in cGMP mTeSR™1 retain the prominent nucleoli and high nuclear-to-cytoplasmic ratio characteristic of this cell type after 10 passages. Densely packed cells and multi-layering are prominent when cells are ready to be passaged.
Figure 2. High Expansion Rates are Observed in cGMP mTeSR™1 Cultures
Graph shows the average fold expansion per passage +/- SEM obtained for hES (H1 and H9) and hiPS (WLS-1C) cells cultured in cGMP mTeSR™1 (red) or non-cGMP mTeSR™1 (gray) on Corning® Matrigel® Matrix over 10 passages. Expansion was determined by enumerating the cell aggregates obtained at harvest and dividing by the number of cell aggregates seeded. Note that this data is representative of cultures passaged after 6-7 days in culture, lower expansion should be expected if using shorter culture times.
Figure 3. Cells Cultured in cGMP mTeSR™1 Medium Express Undifferentiated Cell Markers
Histogram analysis for hES (H1 and H9) and hiPS (WLS-1C) cells characterized using FACS for undifferentiated cell markers, OCT4 (OCT3) (Catalog #60093) and TRA-1-60 (Catalog #60064), after 8 - 10 passages in cGMP mTeSR™1 (filled = sample, blank = isotype control).
Figure 4. hPSCs Maintained in cGMP mTeSR™1 Display a Normal Karyotype
Karyograms of (A) H1 hES and (B) WLS-1C hiPS cells cultured in cGMP mTeSR™1 for 11 passages shows that a normal karyotype is retained.
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| 物种 | 人 |
|---|---|
| 配方 | 无血清 |
cGMP级、无酶的人多能干细胞选择与传代试剂
成分明确的用于人ES和iPS细胞单细胞克隆生成的补充剂
成分明确的无异源基质,支持人多能干细胞在无血清、无饲养层条件下的生长和分化。
与 TeSR™ 维持培养基配合使用,用于维持人胚胎干细胞(ES)和诱导多能干细胞(iPS)的基质
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