EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #03434_C
含重组细胞因子（包括EPO）的甲基纤维素培养基，用于小鼠细胞
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总览
MethoCult™GF M3434针对在小鼠骨髓、脾脏、外周血和胎肝细胞集落形成单位（CFU）检测中造血祖细胞的生长和计数进行优化。MethoCult™GF M3434的配方支持原始红系祖细胞（BFU-E）、粒细胞-巨噬细胞祖细胞（CFU-GM、CFU-G和CFU-M）和多潜能的粒细胞-红系-巨噬细胞-巨核细胞祖细胞（CFU-GEMM）的最佳生长。该配方与STEMvision™软件兼容，用于小鼠骨髓CFU检测的自动集落计数。
参阅关于进行CFU实验的常见问题和解答（FAQ）。
包含
• 含甲基纤维素的Iscove's MDM
• 胎牛血清
• 牛血清白蛋白
• 重组人胰岛素
• 人转铁蛋白（铁饱和）
• 2-巯基乙醇
• 重组小鼠干细胞因子 (SCF)
• 重组小鼠白细胞介素3 (IL-3)
• 重组人白细胞介素6 (IL-6)
• 重组人促红细胞生成素 (EPO)
• 添加物
分类
半固体培养基，专用培养基
细胞类型
造血干/祖细胞
种属
小鼠
应用
细胞培养，克隆筛选，功能学筛选
品牌
MethoCult
研究领域
药物发现和毒性检测，干细胞生物学
制剂类别
甲基纤维素基
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (8)
常见问题
文献 (194)
Pax7 is required for the specification of myogenic satellite cells.
Cell 2000 SEP
Abstract
The paired box transcription factor Pax7 was isolated by representational difference analysis as a gene specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization revealed that Pax7 was also expressed in satellite cells residing in adult muscle. Cell culture and electron microscopic analysis revealed a complete absence of satellite cells in Pax7(-/-) skeletal muscle. Surprisingly,fluorescence-activated cell sorting analysis indicated that the proportion of muscle-derived stem cells was unaffected. Importantly,stem cells from Pax7(-/-) muscle displayed almost a 10-fold increase in their ability to form hematopoietic colonies. These results demonstrate that satellite cells and muscle-derived stem cells represent distinct cell populations. Together these studies suggest that induction of Pax7 in muscle-derived stem cells induces satellite cell specification by restricting alternate developmental programs.
Thermal injury increases the number of eosinophil progenitors in rat spleen and bone marrow.
Inflammation 2001 OCT
Abstract
We have investigated the effects of thermal injury upon myelopoiesis. IL-3,GM-CSF,and IL-5 were used to stimulate myeloid colony formation. IL-3 induces early myeloid progenitors and a more developed myeloid progenitor,the granulocyte-macrophage colony-forming unit (GM-CFU),to multiply and develop into mature myeloid cells. GM-CSF induces GM-CFU to become mature myeloid cells,while IL-5 induces eosinophil progenitors to become mature eosinophils. Stem Cell Factor (SCF) + IL-6 and FLT3 ligand,which have no effect on colony formation by themselves,were used to enhance the effects of IL-3 and GM-CSF,respectively. We found that thermal injury increased the number of early myeloid progenitors and GM-CFU in the spleen with either IL-3 or GM-CSF as a stimulant. Thermal injury increased the number of early myeloid progenitors in the bone marrow when GM-CSF,but not IL-3,was used to stimulate colony growth. Also,thermal injury increased the numbers of eosinophil progenitors in rat spleen and bone marrow and increased splenic levels of IL-5 mRNA.
Hematopoietic stem cells differentiate into vascular cells that participate in the pathogenesis of atherosclerosis.
Nature medicine 2002 APR
Abstract
Excessive accumulation of smooth-muscle cells (SMCs) has a key role in the pathogenesis of vascular diseases. It has been assumed that SMCs derived from the outer medial layer migrate,proliferate and synthesize extracellular matrix components on the luminal side of the vessel. Although much effort has been devoted to targeting migration and proliferation of medial SMCs,there is no effective therapy that prevents occlusive vascular remodeling. We show here that in models of post-angioplasty restenosis,graft vasculopathy and hyperlipidemia-induced atherosclerosis,bone-marrow cells give rise to most of the SMCs that contribute to arterial remodeling. Notably,purified hematopoietic stem cells differentiate into SMCs in vitro and in vivo. Our findings indicate that somatic stem cells contribute to pathological remodeling of remote organs,and may provide the basis for the development of new therapeutic strategies for vascular diseases through targeting mobilization,homing,differentiation and proliferation of bone marrow-derived vascular progenitor cells.
更多信息
| 物种 | 小鼠 |
|---|---|
| Contains | • Methylcellulose in Iscove's MDM • Fetal bovine serum • Bovine serum albumin • Recombinant human insulin • Human transferrin (iron-saturated) • 2-Mercaptoethanol • Recombinant mouse stem cell factor (SCF) • Recombinant mouse interleukin 3 (IL-3) • Recomb |
| 配方 | 甲基纤维素基 |
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