ImmunoCult™ 人B细胞扩增试剂盒

扩增B细胞的无血清培养试剂盒

只有 %1 左

率先评论此产品

¥9,654.00

产品号 #（选择产品）

产品号 #100-0645_C

扩增B细胞的无血清培养试剂盒

产品优势

无需使用血清、饲养细胞或专用培养板，即可在体外高效扩增人类B细胞
无血清培养基和无动物源成分添加剂

产品组分包括

ImmunoCult™-XF B细胞基础培养基，100mL
ImmunoCult™-ACF人B细胞扩增添加剂，2mL

立即询价

总览

无需使用血清、饲养层细胞或专用培养板，即可实现人B细胞的稳定扩增用于下游检测和应用。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基，两者共同作用实现人B细胞的持续激活和扩增，并使其成熟为浆细胞。仅需一周或更短时间的培养，即可收获B细胞并直接用于下游应用。为了方便起见，试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如，您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞，记忆B细胞和初始B细胞，分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

实验数据

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

文献 (2)

Integrating single-cell RNA and T cell/B cell receptor sequencing with mass cytometry reveals dynamic trajectories of human peripheral immune cells from birth to old age Y. Wang et al. Nature Immunology 2025 Jan

Abstract

A comprehensive understanding of the evolution of the immune landscape in humans across the entire lifespan at single-cell transcriptional and protein levels,during development,maturation and senescence is currently lacking. We recruited a total of 220 healthy volunteers from the Shanghai Pudong Cohort (NCT05206643),spanning 13 age groups from 0 to over 90 years,and profiled their peripheral immune cells through single-cell RNA-sequencing coupled with single T cell and B cell receptor sequencing,high-throughput mass cytometry,bulk RNA-sequencing and flow cytometry validation experiments. We revealed that T cells were the most strongly affected by age and experienced the most intensive rewiring in cell–cell interactions during specific age. Different T cell subsets displayed different aging patterns in both transcriptomes and immune repertoires; examples included GNLY+CD8+ effector memory T cells,which exhibited the highest clonal expansion among all T cell subsets and displayed distinct functional signatures in children and the elderly; and CD8+ MAIT cells,which reached their peaks of relative abundance,clonal diversity and antibacterial capability in adolescents and then gradually tapered off. Interestingly,we identified and experimentally verified a previously unrecognized ‘cytotoxic’ B cell subset that was enriched in children. Finally,an immune age prediction model was developed based on lifecycle-wide single-cell data that can evaluate the immune status of healthy individuals and identify those with disturbed immune functions. Our work provides both valuable insights and resources for further understanding the aging of the immune system across the whole human lifespan. In this Resource,authors profile peripheral immune cells from a total of 220 healthy volunteers from birth to over 90 years. This revealed that T cells were most affected by aging with divergent aging patterns in different subsets and identified a population of cytotoxic B cells that were enriched in children.

View publication

Isolation of a Monoclonal Human scFv Against Cytomegalovirus pp71 Antigen Using Yeast Display K. Aoki et al. Antibodies 2025 Jul

Abstract

Background: Human cytomegalovirus (CMV) is a major pathogen that poses significant risks to immunocompromised individuals and neonates. The tegument protein pp71,encoded by the UL82 gene,plays a pivotal role in initiating viral lytic replication and evading host immune responses. Despite its clinical relevance,standardized monoclonal antibodies (mAbs) for pp71 remain limited,prompting the need to expand the available repertoire of antibodies targeting this critical protein. Methods: In this study,we constructed a diverse human single-chain variable fragment (scFv) library using RNA derived from the B cells of four healthy donors. The library was expressed in Saccharomyces cerevisiae,and iterative rounds of magnetic-activated cell sorting (MACS) were performed against recombinant pp71. Clonal enrichment was monitored using flow cytometry. Results: Among the isolated clones,one designated ID2 exhibited high sensitivity and specificity for pp71,as demonstrated by flow cytometry,immunofluorescence,an enzyme-linked immunosorbent assay (ELISA),and biolayer interferometry (BLI). Conclusions: Collectively,these findings establish a novel pp71-specific mAb and underscore the utility of yeast surface display combined with MACS for expanding the antibody toolkit available for CMV research and diagnostics.

View publication

View All Publications

更多信息
物种	人
配方	无血清

更多信息

物种

人

配方

无血清

ImmunoCult™ 人B细胞扩增试剂盒

产品号 #100-0645

产品号 #（选择产品）

产品号 #100-0645_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

Request Pricing

更多信息

Scientists Helping Scientists™

ImmunoCult™ 人B细胞扩增试剂盒

产品号 #100-0645

产品号 #（选择产品）

产品号 #100-0645_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (9)

文献 (2)

Abstract

Abstract

Request Pricing

更多信息

欢迎您关注STEMCELL Technologies的微信公众平台