EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05630_C
用于培养人乳腺上皮细胞
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总览
EpiCult™-C 培养基（人）是一款无血清培养基，专为短期培养人乳腺腔细胞和肌上皮细胞而优化。添加胶原酶/透明质酸酶（产品号 #07912）后，该培养基也非常适合酶解人乳腺组织。
本试剂盒包含 EpiCult™-C 基础培养基（人）和 EpiCult™-C 增殖添加物（人）。需添加氢化可的松原液（产品号 #07925）。
分类
专用培养基
细胞类型
乳腺细胞
种属
人
应用
培养
品牌
EpiCult
研究领域
上皮细胞研究
制剂类别
无血清
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
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相关材料与文献
技术资料 (6)
文献 (2)
High incidence and geographic distribution of cleft palate in Finland are associated with the IRF6 gene
Nature Communications 2024 Nov
Abstract
In Finland,the frequency of isolated cleft palate (CP) is higher than that of isolated cleft lip with or without cleft palate (CL/P). This trend contrasts to that in other European countries but its genetic underpinnings are unknown. We conducted a genome-wide association study in the Finnish population and identified rs570516915,a single nucleotide polymorphism highly enriched in Finns,as strongly associated with CP (P?=?5.25 × 10?34,OR?=?8.65,95% CI 6.11–12.25),but not with CL/P (P?=?7.2 × 10?5),with genome-wide significance. The risk allele frequency of rs570516915 parallels the regional variation of CP prevalence in Finland,and the association was replicated in independent cohorts of CP cases from Finland (P?=?8.82 × 10?28) and Estonia (P?=?1.25 × 10?5). The risk allele of rs570516915 alters a conserved binding site for the transcription factor IRF6 within an enhancer (MCS-9.7) upstream of the IRF6 gene and diminishes the enhancer activity. Oral epithelial cells derived from CRISPR-Cas9 edited induced pluripotent stem cells demonstrate that the CP-associated allele of rs570516915 concomitantly decreases the binding of IRF6 and the expression level of IRF6,suggesting impaired IRF6 autoregulation as a molecular mechanism underlying the risk for CP. Here,the authors perform a genome-wide study and identify a genetic variant enriched in the Finnish population that is strongly associated with isolated cleft palate. This finding suggests a genetic basis for the high prevalence of cleft palate in Finland.
Identification of functional non-coding variants associated with orofacial cleft
Nature Communications 2025 Jul
Abstract
Oral facial cleft (OFC) comprises cleft lip with or without cleft palate (CL/P) or cleft palate only. Genome wide association studies (GWAS) of isolated OFC have identified common single nucleotide polymorphisms (SNPs) in many genomic loci where the presumed effector gene (for example,IRF6 in the 1q32 locus) is expressed in embryonic oral epithelium. To identify candidates for functional SNPs at eight such loci we conduct a massively parallel reporter assay in a fetal oral epithelial cell line,revealing SNPs with allele-specific effects on enhancer activity. We filter these SNPs against chromatin-mark evidence of enhancers and test a subset in traditional reporter assays,which support the candidacy of SNPs at loci containing FOXE1, IRF6, MAFB, TFAP2A,and TP63. For two SNPs near IRF6 and one near FOXE1,we engineer the genome of induced pluripotent stem cells,differentiate the cells into embryonic oral epithelium,and discover allele-specific effects on the levels of effector gene expression,and,in two cases,the binding affinity of transcription factors FOXE1 or ETS2. Conditional analyses of GWAS data suggest the two functional SNPs near IRF6 account for the majority of risk for CL/P at this locus. This study connects genetic variation associated with OFC to mechanisms of pathogenesis. Non-syndromic orofacial cleft is a relatively common congenital anomaly. Many non-coding genetic variants are associated with this disorder but only a subset is functional. Here the authors use reporter assays and stem cells to reveal members of this subset.
更多信息
| 物种 | 人 |
|---|---|
| 配方 | 无血清 |
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