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EasySep™ 小鼠F4/80正选试剂盒

从小鼠脾脏、腹腔灌洗液、肺和肝组织样本中通过免疫磁珠正选分离小鼠F4/80+细胞
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产品号 #（选择产品）

产品号 #100-0659_C

从小鼠脾脏、腹腔灌洗液、肺和肝组织样本中通过免疫磁珠正选分离小鼠F4/80+细胞

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达90%
  • 分选得到的细胞无荧光标记

产品组分包括

  • EasySep™小鼠F4/80正选抗体混合物组分A，0.3 mL
  • EasySep™小鼠F4/80正选抗体混合物组分B，0.6 mL
  • EasySep™ Dextran RapidSpheres™ 50100 磁珠，2 × 1 mL
  • 小鼠FcR多克隆阻断剂（产品号 #300-0902），1.2 mL
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  1. EasySep™磁极
    EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™小鼠F4/80正选试剂盒，通过免疫磁珠正选，从小鼠脾脏、腹腔灌洗液、肺和肝组织样本中轻松分离高纯度的小鼠F4/80+细胞。EasySep™在已发表的研究中被广泛使用了20多年，其将单克隆抗体的特异性与无柱磁珠分选系统的简便性结合在一起。

在这个EasySep™正选流程中，目标细胞被识别F4/80的抗体复合物和磁珠标记。使用EasySep™磁极，只需倾倒或吸出非目的细胞即可分离被标记的细胞。目标细胞保留在试管中。在短至24分钟的磁珠分选后，所需的F4/80+细胞即可用于下游应用，如流式细胞术、细胞培养和基于细胞的实验。

了解更多关于EasySep™免疫磁珠技术的工作原理。探索为您工作流程优化的其他产品，包括培养基、添加物、抗体等。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• EasyPlate™ EasySep™磁极（产品号 #18102）
• EasyEights™ EasySep™磁极（产品号 #18103）
 
分类
细胞分选试剂盒
 
细胞类型
巨噬细胞
 
种属
小鼠
 
样本来源
肺，其他组织，脾脏
 
分选方法
正选
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫，感染性疾病(传染病)
 

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实验数据

FACS plot showing cell populations from lung tissue pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 1. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Lung Tissue

Starting with a naïve mouse lung single-cell suspension, the F4/80+ cell content of the isolated fraction is typically 94.3 ± 2.8% (mean ± SD) using the purple EasySep™ magnet. In the above example, the purities of the start and final isolated fractions are 26.3% and 95.3%, respectively.

Typical Profile for F4/80+ Cells Isolated from Peritoneal Lavage Using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 2. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Peritoneal Lavage

Starting with lavage cells from the peritoneal cavity of a naïve mouse, the F4/80+ cell content of the isolated fraction is typically 97.0 ± 0.4% (mean ± SD) using the purple EasySep™ magnet. In the above example, the purities of the start and final isolated fractions are 47.9% and 97.0%, respectively.

FACS plot showing cell populations from spleen pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 3. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Spleen

Starting with naïve mouse splenocytes, the F4/80+ cell content of the isolated fraction is typically 88.8 ± 3.4% (mean ± SD) using the purple EasySep™ magnet. In the above example, the purities of the start and final isolated fractions are 5.4% and 90.6%, respectively.

Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Liver

Figure 4. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Liver

(A) Starting with manually processed naïve mouse liver single-cell suspensions, the F4/80+ cell content of the isolated fraction is typically 85.2 ± 5.0% (mean ± SD) using the purple EasySep™ Magnet. In the above example, the purities of the start and final isolated fractions are 23.9% and 88.6%, respectively. (B) Starting with naïve mouse liver single-cell suspensions processed using the automated STEMprep™ Tissue Dissociator and STEMprep™ Mouse Liver Dissociation Kit, the F4/80+ cell content of the isolated fraction is typically 94.4 ± 3.8% (mean ± SD) using the purple EasySep™ Magnet. In the above example, the purities of the start and final isolated fractions are 54.3% and 96.6%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
产品说明书 - 脾脏
Product Name
EasySep™ 小鼠F4/80正选试剂盒
Catalog #
100-0659
Lot #
1000158054 or higher
Language
中文
Document Type
产品说明书 - 肺
Product Name
EasySep™ 小鼠F4/80正选试剂盒
Catalog #
100-0659
Lot #
1000158054 or higher
Language
中文
Document Type
Product Information Sheet - Spleen
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Product Information Sheet - Peritoneal Lavage
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Product Information Sheet - Liver
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Product Information Sheet - Lung Tissue
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (11)

How to Dissociate Mouse Lung into a Single-Cell Suspension
实验方案
How to Dissociate Mouse Lung into a Single-Cell Suspension
How to Isolate Mouse Peritoneal Cells
实验方案
How to Isolate Mouse Peritoneal Cells
How to Manually Dissociate Mouse Liver Tissue into a Single-Cell Suspension
实验方案
How to Manually Dissociate Mouse Liver Tissue into a Single-Cell Suspension
A Serum-Free Medium for Differentiation of Monocytes to Macrophages
科学海报
A Serum-Free Medium for Differentiation of Monocytes to Macrophages
Easy Isolation of F4/80-Positive Macrophages from Mouse Tissues
科学海报
Easy Isolation of F4/80-Positive Macrophages from Mouse Tissues
Mouse Immune Cell Isolation Course
点播课程
Mouse Immune Cell Isolation Course
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
视频
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
视频
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Online Immunology Journal Club: New Strategies to Target Macrophages in Cancer
视频
Online Immunology Journal Club: New Strategies to Target Macrophages in Cancer
Frequencies and Percentages of Mouse Immune Cell Types
挂图
Frequencies and Percentages of Mouse Immune Cell Types
Streamline Your Tissue Processing Using the Automated STEMprep™ Tissue Dissociator
线上讲座
Streamline Your Tissue Processing Using the Automated STEMprep™ Tissue Dissociator

文献 (9)

Novel lipid emulsion for total parenteral nutrition based on 18-carbon n-3 fatty acids elicits a superior immunometabolic phenotype in a murine model compared with standard lipid emulsions. E. Lucchinetti et al. The American journal of clinical nutrition 2022 dec

Abstract

BACKGROUND While lipid emulsions in modern formulations for total parenteral nutrition (TPN) provide essential fatty acids and dense calories,they also promote inflammation and immunometabolic disruptions. OBJECTIVES We aimed to develop a novel lipid emulsion for TPN use with superior immunometabolic actions compared with available standard lipid emulsions. METHODS A novel lipid emulsion [Vegaven (VV)] containing 30% of 18-carbon n-3 fatty acids ($\alpha$-linolenic acid and stearidonic acid) was developed for TPN (VV-TPN) and compared with TPN containing soybean oil-based lipid emulsion (IL-TPN) and fish-oil-based lipid emulsion (OV-TPN). In vivo studies were performed in instrumented male C57BL/6 mice subjected to 7-d TPN prior to analysis of cytokines,indices of whole-body and hepatic glucose metabolism,immune cells,lipid mediators,and mucosal bowel microbiome. RESULTS IL-6 to IL-10 ratios were significantly lower in liver and skeletal muscle of VV-TPN mice when compared with IL-TPN or OV-TPN mice. VV-TPN and OV-TPN each increased hepatic insulin receptor abundance and resulted in similar HOMA-IR values,whereas only VV-TPN increased hepatic insulin receptor substrate 2 and maintained normal hepatic glycogen content,effects that were IL-10-dependent and mediated by glucokinase activation. The percentages of IFN-$\gamma$- and IL-17-expressing CD4+ T cells were increased in livers of VV-TPN mice,and liver macrophages exhibited primed phenotypes when compared with IL-TPN. This immunomodulation was associated with successful elimination of the microinvasive bacterium Akkermansia muciniphila from the bowel mucosa by VV-TPN as opposed to standard lipid emulsions. Assay of hepatic lipid mediators revealed a distinct profile with VV-TPN,including increases in 9(S)-hydroxy-octadecatrienoic acid. When co-administered with IL-TPN,hydroxy-octadecatrienoic acids mimicked the VV-TPN immunometabolic phenotype. CONCLUSIONS We here report the unique anti-inflammatory,insulin-sensitizing,and immunity-enhancing properties of a newly developed lipid emulsion designed for TPN use based on 18-carbon n-3 fatty acids.
View publication
Identification of miRNAs that target Fcγ receptor-mediated phagocytosis during macrophage activation syndrome Frontiers in Immunology 2024 Mar

Abstract

Macrophage activation syndrome (MAS) is a life-threatening complication of systemic juvenile arthritis,accompanied by cytokine storm and hemophagocytosis. In addition,COVID-19–related hyperinflammation shares clinical features of MAS. Mechanisms that activate macrophages in MAS remain unclear. Here,we identify the role of miRNA in increased phagocytosis and interleukin-12 (IL-12) production by macrophages in a murine model of MAS. MAS significantly increased F4/80+ macrophages and phagocytosis in the mouse liver. Gene expression profile revealed the induction of Fcγ receptor–mediated phagocytosis (FGRP) and IL-12 production in the liver. Phagocytosis pathways such as High-affinity IgE receptor is known as Fc epsilon RI -signaling and pattern recognition receptors involved in the recognition of bacteria and viruses and phagosome formation were also significantly upregulated. In MAS,miR-136-5p and miR-501-3p targeted and caused increased expression of Fcgr3,Fcgr4,and Fcgr1 genes in FGRP pathway and consequent increase in phagocytosis by macrophages,whereas miR-129-1-3p and miR-150-3p targeted and induced Il-12. Transcriptome analysis of patients with MAS revealed the upregulation of FGRP and FCGR gene expression. A target analysis of gene expression data from a patient with MAS discovered that miR-136-5p targets FCGR2A and FCGR3A/3B,the human orthologs of mouse Fcgr3 and Fcgr4,and miR-501-3p targets FCGR1A,the human ortholog of mouse Fcgr1. Together,we demonstrate the novel role of miRNAs during MAS pathogenesis,thereby suggesting miRNA mimic–based therapy to control the hyperactivation of macrophages in patients with MAS as well as use overexpression of FCGR genes as a marker for MAS classification.
View publication
Caspase-11 mediated inflammasome activation in macrophages by systemic infection of International Journal of Oral Science 2024 Aug

Abstract

Clinical studies have shown that Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is associated with aggressive periodontitis and can potentially trigger or exacerbate rheumatoid arthritis (RA). However,the mechanism is poorly understood. Here,we show that systemic infection with A. actinomycetemcomitans triggers the progression of arthritis in mice anti-collagen antibody-induced arthritis (CAIA) model following IL-1β secretion and cell infiltration in paws in a manner that is dependent on caspase-11-mediated inflammasome activation in macrophages. The administration of polymyxin B (PMB),chloroquine,and anti-CD11b antibody suppressed inflammasome activation in macrophages and arthritis in mice,suggesting that the recognition of lipopolysaccharide (LPS) in the cytosol after bacterial degradation by lysosomes and invasion via CD11b are needed to trigger arthritis following inflammasome activation in macrophages. These data reveal that the inhibition of caspase-11-mediated inflammasome activation potentiates aggravation of RA induced by infection with A. actinomycetemcomitans. This work highlights how RA can be progressed by inflammasome activation as a result of periodontitis-associated bacterial infection and discusses the mechanism of inflammasome activation in response to infection with A. actinomycetemcomitans.
View publication
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更多信息

更多信息
物种 小鼠
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog #18102) • EasyEights™ EasySep™ Magnet (Catalog #18103)
样本来源 其它细胞系, 肺, 脾脏
Selection Method Positive
标记抗体
  1. 抗小鼠CD45抗体，clone 30-F11
    抗小鼠CD45抗体，clone 30-F11

    大鼠Monoclonal IgG2b抗体，抗小鼠CD45

  2. 抗小鼠F4/80抗体，clone BM8
    抗小鼠F4/80抗体，clone BM8

    大鼠Monoclonal IgG2a抗体，抗小鼠F4/80

  3. 抗小鼠CD11b抗体，clone M1/70
    抗小鼠CD11b抗体，clone M1/70

    抗人、小鼠、恒河猴CD11b的大鼠Monoclonal IgG2b抗体

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