EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #100-0691_C
用于提高人胚胎干细胞和诱导多能干细胞在单细胞工作流程中存活率的添加物
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系。
总览
使用 CloneR™2可生成可保持基因组完整性和下游分化潜能的人多能干细胞 (hPSC) 克隆系。可在各种高应激条件下（包括高密度或低密度接种）显著提高人胚胎干细胞（ES）和诱导多能干细胞（iPS）的克隆效率和存活率。
在您的基因编辑流程中，添加 CloneR™2 可提升 ES 和 iPS 细胞电转后以及单细胞沉降（Single-cell deposition）的存活率（参见下文数据）。该添加物还可促进低密度接种（<25 个细胞/cm²）或 FACS 单细胞分选（1 个细胞/孔）获得的克隆细胞的存活和扩增，无需进行单细胞适应过程。同时，CloneR™2 也适用于在较高密度铺板的单细胞存活，包括诸如冻存复苏、或分化前建立单层细胞等应激条件下的应用。
CloneR™2 是一种成分明确、无血清的添加物，可与 TeSR™ ES 和 iPS 细胞培养基兼容使用，并适用于您选择的细胞培养基质。
分类
添加剂
细胞类型
多能干细胞
种属
人
应用
细胞培养
品牌
CloneR
研究领域
细胞系制备，疾病建模，干细胞生物学
制剂类别
无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (16)
文献 (10)
Dynein and dynactin move long-range but are delivered separately to the axon tip
The Journal of Cell Biology 2024 Feb
Abstract
Fellows et al. report that individual dynein motors can move the entire axon length during retrograde transport. They find factors LIS1 and NDEL1,needed for transport initiation,also move with cargos. In the anterograde direction,dynein and its cofactor dynactin are transported separately,keeping them apart until required. Axonal transport is essential for neuronal survival. This is driven by microtubule motors including dynein,which transports cargo from the axon tip back to the cell body. This function requires its cofactor dynactin and regulators LIS1 and NDEL1. Due to difficulties imaging dynein at a single-molecule level,it is unclear how this motor and its regulators coordinate transport along the length of the axon. Here,we use a neuron-inducible human stem cell line (NGN2-OPTi-OX) to endogenously tag dynein components and visualize them at a near-single molecule regime. In the retrograde direction,we find that dynein and dynactin can move the entire length of the axon (>500 µm). Furthermore,LIS1 and NDEL1 also undergo long-distance movement,despite being mainly implicated with the initiation of dynein transport. Intriguingly,in the anterograde direction,dynein/LIS1 moves faster than dynactin/NDEL1,consistent with transport on different cargos. Therefore,neurons ensure efficient transport by holding dynein/dynactin on cargos over long distances but keeping them separate until required.
The immunopathological landscape of human pre-TCRα deficiency: from rare to common variants
Science (New York,N.Y.) 2024 Mar
Abstract
We describe humans with rare biallelic loss-of-function PTCRA variants impairing pre-TCRα expression. Low circulating naïve αβ T cell counts at birth persisted over time,with normal memory αβ and high γδ T cell counts. Their TCRα repertoire was biased,suggesting that noncanonical thymic differentiation pathways can rescue αβ T cell development. Only a minority of these individuals were sick,with infection,lymphoproliferation,and/or autoimmunity. We also report that 1 in 4000 individuals from the Middle East and South Asia are homozygous for a common hypomorphic PTCRA variant. They had normal circulating naïve αβ T cell counts but high γδ T cell counts. Although residual pre-TCRα expression drove the differentiation of more αβ T cells,autoimmune conditions were more frequent in these patients than in the general population.
protoSpaceJAM: an open-source, customizable and web-accessible design platform for CRISPR/Cas insertional knock-in
Nucleic Acids Research 2024 Jun
Abstract
AbstractCRISPR/Cas-mediated knock-in of DNA sequences enables precise genome engineering for research and therapeutic applications. However,designing effective guide RNAs (gRNAs) and homology-directed repair (HDR) donors remains a bottleneck. Here,we present protoSpaceJAM,an open-source algorithm to automate and optimize gRNA and HDR donor design for CRISPR/Cas insertional knock-in experiments,currently supporting SpCas9,SpCas9-VQR and enAsCas12a Cas enzymes. protoSpaceJAM utilizes biological rules to rank gRNAs based on specificity,distance to insertion site,and position relative to regulatory regions. protoSpaceJAM can introduce ‘recoding’ mutations (silent mutations and mutations in non-coding sequences) in HDR donors to prevent re-cutting and increase knock-in efficiency. Users can customize parameters and design double-stranded or single-stranded donors. We validated protoSpaceJAM’s design rules by demonstrating increased knock-in efficiency with recoding mutations and optimal strand selection for single-stranded donors. An additional module enables the design of genotyping primers for deep sequencing of edited alleles. Overall,protoSpaceJAM streamlines and optimizes CRISPR knock-in experimental design in a flexible and modular manner to benefit diverse research and therapeutic applications. protoSpaceJAM is available open-source as an interactive web tool at protospacejam.czbiohub.org or as a standalone Python package at github.com/czbiohub-sf/protoSpaceJAM. Graphical Abstract Graphical Abstract
更多信息
| 物种 | 人 |
|---|---|
| 配方 | 无血清 |
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