EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #75003_C
细胞增殖和追踪染料
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总览
CFDA-SE（5(6)-羧基荧光素二乙酸琥珀酰亚胺酯）是一种稳定的、可渗透细胞的CFSE的二乙酸前体。在CFDA-SE扩散进入细胞后，细胞内的酯酶裂解乙酸基生成CFSE，而CFSE通过其琥珀酰亚胺基与细胞的胺类相互作用，生成一种不能透过细胞膜的强荧光绿色染料。CFDA-SE常用于细胞增殖试验，因为它会被大致均匀地划分到细胞的后代中，因此可以通过多代分裂过程中荧光强度的连续减半来追踪细胞分裂。CFDA-SE还用于运动测定和体内细胞追踪实验。被CFDA-SE标记的细胞可以用任何与荧光素检测兼容的仪器/滤光片组进行检测。
别名
5(6)-羧基荧光素琥珀酰亚胺酯；6-{[(2，5-二氧代-1-吡咯烷基)氧基]羰基}-3-氧代-3H-螺[2-苯并呋喃-1，9'-呫吨]-3'，6'-二基二乙酸酯；羧基荧光素二乙酸酯琥珀酰亚胺酯；CFSE
细胞类型
其他物种
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
流式细胞术，免疫细胞化学，免疫荧光
研究领域
免疫，神经科学，干细胞生物学
CAS 编号
150347-59-4
化学式
C₂₉H₁₉NO₁₁
分子量
557.5 g/mol
实验数据
产品说明书及文档
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应用领域
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相关材料与文献
技术资料 (1)
文献 (1)
TLR9 agonism differentially impacts human NK cell-mediated direct killing and antibody-dependent cell-mediated cytotoxicity
Scientific Reports 2024 Jun
Abstract
There are two known mechanisms by which natural killer (NK) cells recognize and kill diseased targets: (i) direct killing and (ii) antibody-dependent cell-mediated cytotoxicity (ADCC). We investigated an indirect NK cell activation strategy for the enhancement of human NK cell killing function. We did this by leveraging the fact that toll-like receptor 9 (TLR9) agonism within pools of human peripheral blood mononuclear cells (PBMCs) results in a robust interferon signaling cascade that leads to NK cell activation. After TLR9 agonist stimulation,NK cells were enriched and incorporated into assays to assess their ability to kill tumor cell line targets. Notably,differential impacts of TLR9 agonism were observed—direct killing was enhanced while ADCC was not increased. To ensure that the observed differential effects were not attributable to differences between human donors,we recapitulated the observation using our Natural Killer—Simultaneous ADCC and Direct Killing Assay (NK-SADKA) that controls for human-to-human differences. Next,we observed a treatment-induced decrease in NK cell surface CD16—known to be shed by NK cells post-activation. Given the essential role of CD16 in ADCC,such shedding could account for the observed differential impact of TLR9 agonism on NK cell-mediated killing capacity.
更多信息
| Molecular Weight | 557.5 g/mol |
|---|---|
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
| Alternative Names | 5(6)-Carboxyfluorescein succinimidyl ester; 6-{[(2, 5-Dioxo-1-pyrrolidinyl)oxy]carbonyl}-3-oxo-3H-spiro[2- benzofuran-1, 9'-xanthene]-3', 6'-diyl diacetate; carboxyfluorescein diacetate succinimidyl ester; CFSE |
| Cas Number | 150347-59-4 |
| Chemical Formula | C₂₉H₁₉NO₁₁ |
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