EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07010_C
防止细胞粘附的培养容器的冲洗液
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总览
原名 AggreWell™ 冲洗液，更名于 2017 年 12 月生效。
抗粘附冲洗液是一种表面活性剂溶液，用于预处理培养器皿，以降低表面张力并防止细胞粘附。它可用于培养板、培养瓶、移液器、过滤器和其他培养器皿。
以下产品需要使用抗粘附冲洗液：
• AggreWell™ 培养板（例如产品号 #34811）
• 类器官生长培养基，包括 HepatiCult™（产品号 #06030）和 PancreaCult™（产品号 #06040）。
种属
人，小鼠，非人灵长类，其他物种，大鼠
品牌
AggreWell
研究领域
癌症，神经科学，干细胞生物学
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (9)
文献 (24)
Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition
Developmental cell 2024 Feb
Abstract
During kidney development,nephron epithelia arise de novo from fate-committed mesenchymal progenitors through a mesenchymal-to-epithelial transition (MET). Downstream of fate specification,transcriptional mechanisms that drive establishment of epithelial morphology are poorly understood. We used human iPSC-derived renal organoids,which recapitulate nephrogenesis,to investigate mechanisms controlling renal MET. Multi-ome profiling via snRNA-seq and ATAC-seq of organoids identified dynamic changes in gene expression and chromatin accessibility driven by activators and repressors throughout MET. CRISPR interference identified that paired box 8 (PAX8) is essential for initiation of MET in human renal organoids,contrary to in vivo mouse studies,likely by activating a cell-adhesion program. While Wnt/β-catenin signaling specifies nephron fate,we find that it must be attenuated to allow hepatocyte nuclear factor 1-beta (HNF1B) and TEA-domain (TEAD) transcription factors to drive completion of MET. These results identify the interplay between fate commitment and morphogenesis in the developing human kidney,with implications for understanding both developmental kidney diseases and aberrant epithelial plasticity following adult renal tubular injury.
Cortical brain organoid slices (cBOS) for the study of human neural cells in minimal networks
iScience 2024 Mar
Abstract
Brain organoids derived from human pluripotent stem cells are a promising tool for studying human neurodevelopment and related disorders. Here,we generated long-term cultures of cortical brain organoid slices (cBOS) grown at the air-liquid interphase from regionalized cortical organoids. We show that cBOS host mature neurons and astrocytes organized in complex architecture. Whole-cell patch-clamp demonstrated subthreshold synaptic inputs and action potential firing of neurons. Spontaneous intracellular calcium signals turned into synchronous large-scale oscillations upon combined disinhibition of NMDA receptors and blocking of GABA A receptors. Brief metabolic inhibition to mimic transient energy restriction in the ischemic brain induced reversible intracellular calcium loading of cBOS. Moreover,metabolic inhibition induced a reversible decline in neuronal ATP as revealed by ATeam1.03 YEMK . Overall,cBOS provide a powerful platform to assess morphological and functional aspects of human neural cells in intact minimal networks and to address the pathways that drive cellular damage during brain ischemia. Subject areas: Neuroscience,Cellular neuroscience,Stem cells research
Engineering an
Frontiers in Neuroscience 2024 May
Abstract
Understanding the retinogeniculate pathway in vitro can offer insights into its development and potential for future therapeutic applications. This study presents a Polydimethylsiloxane-based two-chamber system with axon guidance channels,designed to replicate unidirectional retinogeniculate signal transmission in vitro. Using embryonic rat retinas,we developed a model where retinal spheroids innervate thalamic targets through up to 6 mm long microfluidic channels. Using a combination of electrical stimulation and functional calcium imaging we assessed how channel length and electrical stimulation frequency affects thalamic target response. In the presented model we integrated up to 20 identical functional retinothalamic neural networks aligned on a single transparent microelectrode array,enhancing the robustness and quality of recorded functional data. We found that network integrity depends on channel length,with 0.5–2 mm channels maintaining over 90% morphological and 50% functional integrity. A reduced network integrity was recorded in longer channels. The results indicate a notable reduction in forward spike propagation in channels longer than 4 mm. Additionally,spike conduction fidelity decreased with increasing channel length. Yet,stimulation-induced thalamic target activity remained unaffected by channel length. Finally,the study found that a sustained thalamic calcium response could be elicited with stimulation frequencies up to 31 Hz,with higher frequencies leading to transient responses. In conclusion,this study presents a high-throughput platform that demonstrates how channel length affects retina to brain network formation and signal transmission in vitro.
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
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