EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07060_C
哺乳动物有核细胞手动计数试剂
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总览
推荐使用含3%乙酸的亚甲基蓝对有核哺乳动物细胞进行手动计数。该试剂会裂解红细胞和白细胞的胞膜。亚甲基蓝会将留下的白细胞核染成浅蓝色。
包含
乙酸 (3%)
亚甲基蓝
蒸馏水
本产品具有危险性。请参阅安全数据表 (SDS)。
细胞类型
其他物种
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
细胞培养
研究领域
免疫，神经科学，干细胞生物学
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (2)
文献 (1)
Protocol for isolation and expansion of natural killer cells from human peripheral blood scalable for clinical applications
Biology Methods & Protocols 2025 Feb
Abstract
AbstractNatural killer (NK) cells have emerged as promising candidates for novel immunotherapy strategies against various malignancies. Their unique ability to recognize and eliminate tumour cells without prior sensitization,coupled with the secretion of pro-inflammatory cytokines such as interferon-gamma and tumour necrosis factor,position them as promising agents in cancer therapy. Adoptive NK cell transfer has shown particular promise in haematological malignancies,where NK cell infusions could achieve remission in a high proportion of patients. Moreover,the possibility to engineer NK cells to express chimeric antigen receptors can further enhance their efficacy,thereby broadening their applicability to include solid tumours. Ongoing research is crucial to optimize NK cell therapies and enhance their efficacy to expand their clinical applications. However,this research hinges on robust protocols and experimental methodology for the isolation,expansion,and genetic engineering of NK cells. In an attempt to set up a standardized protocol for NK cell isolation and expansion,we present a thoroughly tested and validated protocol that can produce highly pure,viable,and potent NK cells that can be used for research and development of NK cell therapies. The protocol is highly reproducible,closely aligned to comply with Good Manufacturing Practice regulations,and tested for scalability to produce NK cells at clinically relevant dosages to support the development of off-the-shelf NK products.
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