EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07820_C
用于分离病毒、细胞器、大分子或细胞的密度梯度离心液
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总览
使用 OptiPrep™ 密度梯度离心液，可简化大分子、病毒、多种细胞类型以及细胞器（例如细胞核、线粒体、内体或外泌体）的分离和纯化。这款灵活、多功能且温和的密度梯度介质基于非离子型碘克沙醇（60% w/v），密度为 1.320 ± 0.001 g/mL。
包含
• Iodixanol（碘克沙醇）：60% (w/v)
• 密度：1.320 ± 0.001 g/mL
细胞类型
单个核细胞，其他物种
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
细胞分选
品牌
OptiPrep
研究领域
免疫
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (1)
文献 (17)
Sea lamprey enlightens the origin of the coupling of retinoic acid signaling to vertebrate hindbrain segmentation
Nature Communications 2024 Feb
Abstract
Retinoic acid (RA) is involved in antero-posterior patterning of the chordate body axis and,in jawed vertebrates,has been shown to play a major role at multiple levels of the gene regulatory network (GRN) regulating hindbrain segmentation. Knowing when and how RA became coupled to the core hindbrain GRN is important for understanding how ancient signaling pathways and patterning genes can evolve and generate diversity. Hence,we investigated the link between RA signaling and hindbrain segmentation in the sea lamprey Petromyzon marinus,an important jawless vertebrate model providing clues to decipher ancestral vertebrate features. Combining genomics,gene expression,and functional analyses of major components involved in RA synthesis (Aldh1as) and degradation (Cyp26s),we demonstrate that RA signaling is coupled to hindbrain segmentation in lamprey. Thus,the link between RA signaling and hindbrain segmentation is a pan vertebrate feature of the hindbrain and likely evolved at the base of vertebrates. Retinoic acid signaling is involved in patterning the embryonic antero-posterior axis,and also regulates hindbrain segmentation in jawed vertebrates. Here they show that retinoic acid signaling plays important roles in hindbrain segmentation in a jawless vertebrate,the lamprey,thus indicating this feature of hindbrain development is conserved in all vertebrates.
Label-free separation of peripheral blood mononuclear cells from whole blood by gradient acoustic focusing
Scientific Reports 2024 Apr
Abstract
Efficient techniques for separating target cells from undiluted blood are necessary for various diagnostic and research applications. This paper presents acoustic focusing in dense media containing iodixanol to purify peripheral blood mononuclear cells (PBMCs) from whole blood in a label-free and flow-through format. If the blood is laminated or mixed with iodixanol solutions while passing through the resonant microchannel,all the components (fluids and cells) rearrange according to their acoustic impedances. Red blood cells (RBCs) have higher effective acoustic impedance than PBMCs. Therefore,they relocate to the pressure node despite the dense medium,while PBMCs stay near the channel walls due to their negative contrast factor relative to their surrounding medium. By modifying the medium and thus tuning the contrast factor of the cells,we enriched PBMCs relative to RBCs by a factor of 3600 to 11,000 and with a separation efficiency of 85%. That level of RBC depletion is higher than most other microfluidic methods and similar to that of density gradient centrifugation. The current acoustophoretic chip runs up to 20 µl/min undiluted whole blood and can be integrated with downstream analysis.
MHC class I-dressing is mediated via phosphatidylserine recognition and is enhanced by polyI:C
iScience 2024 Apr
Abstract
SummaryIn addition to cross-presentation,cross-dressing plays an important role in the induction of CD8+ T cell immunity. In the process of cross-dressing,conventional dendritic cells (DCs) acquire major histocompatibility complex class I (MHCI) from other cells and subsequently prime CD8+ T cells via the pre-formed antigen-MHCI complexes without antigen processing. However,the mechanisms underlying the cross-dressing pathway,as well as the relative contributions of cross-presentation and cross-dressing to CD8+ T cell priming are not fully understood. Here,we demonstrate that DCs rapidly acquire MHCI-containing membrane fragments from dead cells via the phosphatidylserine recognition-dependent mechanism for cross-dressing. The MHCI dressing is enhanced by a TLR3 ligand polyinosinic-polycytidylic acid (polyI:C). Further,polyI:C promotes not only cross-presentation but also cross-dressing in vivo. Taken together,these results suggest that cross-dressing as well as cross-presentation is involved in inflammatory diseases associated with cell death and type I IFN production. Graphical abstract Highlights•Mouse splenic DCs acquire dead cell-derived MHCI via PtdSer recognition•MHCI-containing vesicles are associated with the DC plasma membrane•PolyI:C enhances MHCI dressing•PolyI:C promotes not only cross-presentation but also cross-dressing in vivo Immunology; Components of the immune system; Cell biology
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Contains | • Iodixanol: 60% (w/v) • Density: 1.320 ± 0.001 g/mL |
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