EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #79001_C
用于分子生物学的无DNase/RNase水
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总览
无核酸酶水适用于需要无核酸酶环境的分子生物学实验，包括DNA/RNA相关操作（如PCR、cDNA合成、qPCR等）。本产品经无菌过滤（0.2µm），不含RNase和DNase活性，无内毒素，且未经过DEPC处理。
种属
人，小鼠，大鼠
应用
基因组编辑
研究领域
疾病建模
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
相关材料与文献
技术资料 (3)
文献 (1)
A rapid LAMP assay for the diagnosis of oak wilt with the naked eye
Plant Methods 2024 Aug
Abstract
BackgroundOak wilt disease,caused by Bretziella fagacearum is a significant threat to oak (Quercus spp.) tree health in the United States and Eastern Canada. The disease may cause dramatic damage to natural and urban ecosystems without management. Early and accurate diagnosis followed by timely treatment increases the level of disease control success.ResultsA rapid assay based on loop mediated isothermal amplification (LAMP) was first developed with fluorescence detection of B. fagacearum after 30-minute reaction time. Six different primers were designed to specifically bind and amplify the pathogen’s DNA. To simplify the use of this assay in the field,gold nanoparticles (AuNPs) were designed to bind to the DNA amplicon obtained from the LAMP reaction. Upon inducing precipitation,the AuNP-amplicons settle as a red pellet visible to the naked eye,indicative of pathogen presence. Both infected and healthy red oak samples were tested using this visualization method. The assay was found to have high diagnostic sensitivity and specificity for the B. fagacearum isolate studied. Moreover,the developed assay was able to detect the pathogen in crude DNA extracts of diseased oak wood samples,which further reduced the time required to process samples.ConclusionsIn summary,the LAMP assay coupled with oligonucleotide-conjugated gold nanoparticle visualization is a promising method for accurate and rapid molecular-based diagnosis of B. fagacearum in field settings. The new method can be adapted to other forest and plant diseases by simply designing new primers.Supplementary InformationThe online version contains supplementary material available at 10.1186/s13007-024-01254-8.
更多信息
| 物种 | 人, 大鼠, 小鼠 |
|---|
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